Toxicity to organ cultured heats of media prepared with disposable filter units |
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Authors: | Jacquline R. Wakeland J. Stanley Crie Kern Wildenthal |
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Affiliation: | (1) Pauline and Adolph Weinberger Laboratory for Cardiopulmonary Research, Departments of Physiology and Internal Medicine, The University of Texas Health Science Center at Dallas, 75235 Dallas, Texas |
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Abstract: | Summary Organ cultures of hearts maintained in media sterilized by filtration through different types of commercially available disposable membrane filter units displayed different beating rates and survival patterns. Media filtered through two different filter units sold by one company were severely toxic. Prewashing the filters removed most if not all of the toxins. This work was supported by grants from the National Heart, Lung and Blood Institute (HL14706) and the Moss Heart Fund. |
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Keywords: | membrane toxicity membrane impurities disposable filter units heart cultures |
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