A comparative study of thromboxane and prostacyclin release from ex vivo cultured bovine vascular endothelium |
| |
Authors: | Jonathan C. Goldsmith Samuel W. Needleman |
| |
Affiliation: | Divison of Hematology-Oncology Department of Internal Medicine University of Iowa Iowa City, USA 52242 |
| |
Abstract: | Thromboxane B2, 6-keto-Prostaglandin F1α, and Prostaglandin E2 release have been quantitated from cultured adult by bovine endothelial cell monolayers and from vascular segments employing specific radioimmunoassay and thin layer chromatography. Release of all three prostaglandins was demonstrable from both endothelial cell systems under basal conditions and following exposure to the ionophore A23187 and arachidonic acid. In culture, the quantity of 6-keto-PGF1α released was diminished compared to amounts released from the vessel segments while thromboxane B2 and prostaglandin E2 release were similar in the two endothelial model systems. However, the amount of thromboxane B2 assayed was small and the quantity of thromboxane A2 it represents is probably of little significance to prostacyclin. |
| |
Keywords: | |
本文献已被 ScienceDirect 等数据库收录! |