粘质沙雷氏菌产灵菌红素培养基的筛选

目的：确定菌株S418产生灵菌红素的最优培养基配方及其的分类地位。方法：以花生粉为基础培养基,通过单因素试验和四因素三水平正交试验筛选出了菌株S418产灵菌红素的最佳培养基配方;根据该菌株的16S rRNA基因序列系统发育树分析初步确定了菌株S418的分类地位。结果：培养基最优配方为：花生粉2%,花生油0.5%,L-脯氨酸1%,硫酸镁0.025%。在28℃、pH7.5、250r/min振荡培养24h,灵菌红素产量达67.92mg/L。菌株S418初步鉴定为粘质沙雷氏菌（Serratia marcescensS418）。结论：花生粉培养基是一种适合粘质沙雷氏菌产灵菌红素的优良培养基。

Screening of the Cultivation Medium of Prodigiosin Production from Serratia marcescens

Objective:To determine optimal culture medium and taxonomic status of strain S418 producing prodigiosin.Method: In the base of preliminary determination that peanut powder was the basic medium for fermentable cultivation,the optimal submerged fermentation medium of prodigiosin production for the strain S418 were investigated by single factor test and L_9(3_4) orthogonal test.The strain S418 was identified by 16S ribosomal RNA gene sequences analysis.Result:It showed that the optimal culture was: Peanut powder 2%,peanut oil 0.5%,L-proline 1%,MgSO_4 0.025%.Under fermented condition: culture temperature 28℃,rotation speed 250r/min and fermentation time 24h,the yield of prodigiosin was 67.92mg/L.The strain S418 were preliminarily identified to Serratia marcescens S418.Conclusion: peanut powder broth was a optimal culture medium for prodigiosin production by Serratia marcescens.