A rapid naphthol yellows method for measuring the cellular protein content of anchorage cultures |
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Authors: | Philip Skehan Susan J. Friedman |
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Affiliation: | (1) The Oncology Research Group, Department of Pharmacology, Faculty of Medicine, The University of Calgary, T2N 4N1 Calgary, Alberta, Canada |
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Abstract: | Summary A rapid method has been developed for measuring the cellular protein content of mono- and multilayered anchorage cultures. Fixed or air dried cultures are stained for 30 min with 0.2% Naphthol Yellow S (NYS) dissolved in 1% acetic acid. Unbound dye is removed by a series of four 2.5 min washes in 1% acetic acid, and protein-bound dye extracted with 10 mM unbuffered Tris base for spectrophotometric optical density determination at 433 nm. The NYS method exhibited a least-squares correlation coefficient of 0.99997 with the Oyama-Eagle Lowry method. |
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