Anti-complement activity of polynucleotides. |
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| Authors: | E De Clercq P F Torrence J Hobbs B Janik P De Somer B Witkop |
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| Institution: | 1. Contribution from the Rega Institute for Medical Research University of Leuven, B-3000 Leuven, Belgium;2. The Laboratory of Chemistry, National Institute of Arthritis, Metabolism and Digestive Diseases, National Institutes of Health, Bethesda, Maryland 20014, USA;3. The Max-Planck-Institut fur Experimentelle Medizin, Abteilung Chemie, Gottingen, Germany;4. Molecular Biology Department, Miles Laboratories, Elkhart, Indiana 46514, USA |
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| Abstract: | A biologic assay system, based on complement (C′) inhibition, is described to unravel structural differences among polynucleotides. The C′ system appears particularly suitable to distinguish (1) homo- from copoly-ribonucleotides, (2) deoxyribo- from 2′-OH and other 2′-modified polynucleotides, and (3) single homopolynucleotides from double- or triple-stranded complexes.From these studies a number of polynucleotides emerged with potent anti-C′ activities, worthy of further investigation. The most active polymers were (G)n (polyguanylic acid), (dCc1)n poly(2′-chloro-2′deoxycytidylic acid)] and (dUz)n poly(2′-azido-2′-deoxyuridylic acid)]. |
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