乳腺癌组织中VEGF—C和p38MAPK的表达及与伴淋巴结转移的关系

目的探讨乳腺浸润性导管癌组织中血管内皮生长因子C（VEGF—C）和丝裂原激活蛋白激酶p38（p38MAPK）的表达关系，以及与乳腺浸润性导管癌淋巴结转移等生物学行为的关系。方法采用免疫组织化学sP法检测70例乳腺浸润性导管癌组织及15例癌旁正常组织中VEGF-C和p38MAPK蛋白的表达，并采用Westernblot法检测13例伴有淋巴结转移的乳腺癌及12例无淋巴结转移的乳腺癌的新鲜组织中VEGF—C和p38MAPK蛋白表达。结果VEGF—C和p38MAPK在乳腺浸润性导管癌组织中的表达（阳性率分别为67．0％和61．4％）明显高于癌旁正常组织；VEGF-C和p38MAPK蛋白在伴有淋巴结转移组的乳腺癌组织中的表达均高于无淋巴结转移组（P=0．005，P=0．005）；在乳腺浸润性导管癌组织中VEGF-C和p38MAPK表达存在显著正相关（r=0．383，P=0．001），并与乳腺浸润性导管癌的TNM分期（P=0．019，P=0．010）有关；VEGF-C和p38MAPK蛋白表达与乳腺浸润性导管癌肿块的大小（P=0．203，P=0．086）和患者的年龄（P=0．0．266，P=0．087）无明显关系。Western blot也证实，VEGF-C和p38MAPK蛋白在有淋巴结转移组中表达高于无淋巴结转移组。结论VEGF-C和p38MAPK的蛋白表达与乳腺浸润性导管癌的淋巴结转移密切相关，其有望成为乳腺癌治疗的新靶点。

Relationship between the expression of VEGF-C and p38MAPK and lymphatic metastasis in breast cancer

Objective To investigate the expression of vascular endothelial growth factor C （VEGF- C） and p38MAPK in breast invasive ductal carcinoma （IDC） and the correlation of their expression with lymphatic metastasis and other biological behavior. Methods The expressions of VEGF-C and p38MAPK proteins were examined in 70 paraffin-embedded specimens and 15 adjacent normal tissues by immunohistochemistry, and 13 freshly-taken breast cancer tissues with lymphatic metastasis and 12 without lymphatic metastasis by Western blot. Results The expressions of VEGF-C and p38MAPK proteins in breast cancer tissues were significantly higher than those in the adjacent normal tissues. Their expressions in the lymphatic metastasis group were remarkably higher than in the group without lymphatic metastasis （P~ 0.05）. The expression of VEGF-C was positively related to the expression of p38MAPK （r=0. 383, P= 0. 001）. Their expressions were not related to tumor size and patient, age （P~0.05）. The result of Western blot also showed that their expressions in the metastasis group were higher than in the non-metastasis group. Conclusion The expressions of VEGF-C and p38MAPK are closely related to lymphatic metastasis of breast carcinoma and VEGF-C and p38MAPK may be two new targets in the treatment of breast carcinoma.