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| 正常大鼠胃黏膜中肌球蛋白轻链激酶定性和定位观察 | |
| 引用本文： | 王震,陈浩,邢蓓蓓,黄大可,李响,汪渊,朱华庆,贾雪梅. 正常大鼠胃黏膜中肌球蛋白轻链激酶定性和定位观察[J]. 中国组织化学与细胞化学杂志, 2013, 22(1): 17-19 |
| 作者姓名： | 王震 陈浩 邢蓓蓓 黄大可 李响 汪渊 朱华庆 贾雪梅 |
| 作者单位： | 1. 安徽医科大学第一临床学院,安徽 合肥,230032 2. 安徽医科大学形态学中心实验室,安徽 合肥,230032 3. 安徽医科大学生化教研室,安徽 合肥,230032 |
| 基金项目： | 国家自然科学基金资助，安徽省教育厅自然科学基金资助 |
| 摘 要： | 目的观察正常大鼠胃组织中肌球蛋白轻链激酶的表达及分布特点。方法取11只SD正常雄性大鼠，在饥饿状态下，处死后取胃组织。通过免疫组化染色，观察正常大鼠胃组织中MLCK的表达及分布。结果MLCK在黏膜肌层、肌层和黏膜下层血管壁平滑肌均有大量表达；在胃底腺中，MLCK主要表达于壁细胞和主细胞胞浆内。结论MLCK不仅存在于平滑肌细胞内，还分布于胃底腺壁细胞和主细胞内，可能参与胃底腺腺细胞的分泌活动。 |
| 关 键 词： | MLCK 免疫组织化学 胃 大鼠 |
Study on experssion of MLCK in the gastric mucosa of normal rats |
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| Wang Zhen,Chen Hao,Xing Beibei,Huang Dake,Li Xiang,Wang Yuan,Zhu Huaqing,Jia Xuemei. Study on experssion of MLCK in the gastric mucosa of normal rats[J]. Chinese Journal of Histochemistry and Cytochemistry, 2013, 22(1): 17-19 | |
| Authors: | Wang Zhen Chen Hao Xing Beibei Huang Dake Li Xiang Wang Yuan Zhu Huaqing Jia Xuemei |
| Affiliation: | 1 The First Clinical Collage, 2 Central Morphological Lab, 3 Department of Biochemistry and Molecular Biology, Anhui Medical University, Hefei Anhui 230032,China) |
| Abstract: | Objective To investigate the expression of myosin light chain kinase （MLCK） in the gastric tissue of normal rats. Methods 11 normal rats were killed in a state of starvation. Then the gastric tissue was taken for observation by using immunohistochemical staining. Results A great quantity of MLCK was located in the muscularis mucosas, muscularis and vascular walls of the stomach. Worthy of note was that in the fundie glands of the stomach, MLCK was located in the cytoplasm of gland cells too. Conclusion MLCK is located in not only smooth muscles but also the parietal cells and chief cells in fundic glands of the stomach, being probably closely related to gland cells＇ secretion. |
| Keywords: | MLCK Immunohistochemistry Stomach Rat |
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