Microfluidic device reads up to four consecutive base pairs in DNA sequencing-by-synthesis |
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Authors: | Kartalov Emil P Quake Stephen R |
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Affiliation: | Applied Physics Department, California Institute of Technology, Pasadena, CA 91125, USA. |
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Abstract: | We have developed the first fully integrated microfluidic system for DNA sequencing-by-synthesis. Using this chip and fluorescence detection, we have reliably sequenced up to 4 consecutive bps. The described sequencer can be integrated with other microfluidic components on the same chip to produce true lab-on-a-chip technology. The surface chemistry that was designed to anchor the DNA to elastomeric microchannels is useful in a broad range of studies and applications. |
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