A procedure for the synthesis of uridine-5'-diphospho-N-acetylglucosamine-14C |
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| Authors: | R R Wagner M A Cynkin |
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| Institution: | Tufts University School of Medicine, Department of Biochemistry, 136 Harrison Avenue, Boston, Massachusetts 02111 USA |
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| Abstract: | A complete procedure for the synthesis of 1-14C-glucosamine-labeled UDP-N-acetylglucosamine is described. Glucosamine is first phosphorylated with ATP and hexokinase to form glucosamine 6-phosphate. This is N-acetylated with acetic anhydride, and the product is converted to UDP-N-acetylglucosamine by incubation with a crude yeast extract. The sugar nucleotide is isolated from the incubation mixture by paper electrophoresis, and purified by paper chromatography. |
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