A method of collecting water samples from immediately below an ice cover

A plexiglas box with cellular neoprene gaskets in two opposite faces which can be frozen in situ in a developing ice cover on a lake is described along with a water sampling system using evacuated glass tubes (Vacutainers) and blood collecting needles. This system allows collecting of water samples from directly beneath the ice without disturbing the ice cover. The box may be removed in an ice block at the end of the sampling period.