Nuclear formation in a Drosophila cell-free system |
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| Authors: | M Berrios A A Avilion |
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| Institution: | Department of Pharmacological Sciences, School of Medicine, State University of New York, Stony Brook 11794-8651. |
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| Abstract: | A cell-free preparation obtained from 0- to 5-h-old Drosophila melanogaster embryos induces chromatin decondensation and nuclear formation from demembranated Xenopus sperm. Newly formed nuclei have a peripheral lamina, a double membrane, and structures resembling pore complexes. Indirect immunofluorescence analyses demonstrate the association of Drosophila lamins and DNA topoisomerase II with newly assembled nuclei. |
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