Nitricoxide synthase-induced oxidative stress in prolonged alcoholic myopathies of rats |
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Authors: | Jianfeng Wang Haiying Chu Hua Zhao Xueying Cheng Yanli Liu Wei Jin Jinyao Zhao Bo Liu Yanfang Ding Haiying Ma |
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Institution: | (1) Department of Neurology, Dalian Centre Hospital, Dalian, 116033, Liaoning Province, China;(2) Department of Histology and Embryology, Dalian Medical University, 465 Zhongshan Road, Dalian, 116027, Liaoning Province, China |
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Abstract: | Previous studies showed that nitricoxide synthase (NOS) and oxidative stress can induce skeletal muscle atrophy in the muscular
dystrophy and inclusion-body myopathy. There is a correlation between NOS and oxidative stress. However, it is not clear,
whether there are some changes of the NOS activity in prolonged alcoholic myopathy (PAM), and whether NOS activity has relation
to amyotrophy of PAM. We established experimental alcoholic myopathy model of rats by prolonged alcohol intake. We found that
there is a reduction in GSH-px (P < 0.05) and an increase of SOD (P < 0.05), MDA (P < 0.05) and iNOS (P < 0.05) in the plantaris of the experimental group by spectrophotometer. In the soleus of the experimental group, except
for MDA showed an increase (P < 0.05), the other enzymes showed no obvious difference (P > 0.05). The immunohistochemistry results showed that there was obvious expression of iNOS in the cytoplasm of plantaris
in the experimental group and there was no expression of iNOS in the control group. There was a decrease of nNOS expression
on the membranes of the plantaris cells in the experimental group by immunofluorescence. Meanwhile, we found the expression
of nNOS in some cytoplasm. Our results suggested that NOS might be an important factor during the development of PAM. We could
infer that there are some disturbances with regard to output and scavenging of free radical in PAM. Alcohol can induce the
oxidative stress reaction and further result in imbalance of the oxidant-antioxidant status in the organism.
Haiying Chu is the co-first author. |
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Keywords: | Prolonged alcoholic myopathy Nitricoxide synthase Oxidative stress |
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