谷胱甘肽对顺铂致小鼠染色体畸变的保护作用

为了研究谷胱甘Lk．（GSH）对顺铂（cDDP）所致染色体畸变的影响，将昆明小鼠随机分为空白对照组、GSH组、CDDP组、GSH＋CDDP组进行实验，GSH组按照1200mg／（kg·d1连续3天尾静脉注射GSH，对照组注射等量生理盐水；CDDP组于第2天一次注射顺铂20mg/ｋg。对小鼠尾静脉取血检测血常规，处死小鼠，取骨髓进行有核细胞计数、微核实验和染色体G显带，双盲阅片并进行统计学分析。结果表叽对照组细胞染色体数目与形态完好，微核出现的机率极低。GSH组与空白对照组相比无显著差异（Ｐ〉0．05）。CDDP组、GSH＋CDDP组出现了明显的骨髓抑制现象，染色体畸变率及微核率显著增高，与空白对照组比较均有显著性差异（Ｐ〈0．01）。GSH＋CDDP组与CDDP组相比染色体畸变率明显下降（Ｐ〈0．05）。表明谷胱甘肽对顺铂所致的染色体损伤具有一定的保护作用。

The Protective Effect of Glutathione on Chromosome Aberration of Mice Induced by Cisplatin

To study the effect of glutathione （GSH） on chromosome aberration induced by cisplatin （CDDP）, Kunming mice in the experiment were randomly divided into the control group, GSH group, CDDP group, and group of GSH＋CDDP. The mice of GSH Group were injected with Glutathione by tail vein in accordance with 1 200 mg/（kg-d） in three days. The control group were injected with the same volume of normal saline. The CDDP group were injected once with Cisplatin of 20 mg/kg on the 2nd day. The blood sample was drawn by the tail vein of mice for routine blood test. Kill the mice and get the bone marrows for nucleated cell count, micronucleus test and chromosome G banding. Read the slides with double-blind method and carry out the statistical analysis. The results showed that, for the control group, the numbers and morphology of the chromosome were regular, and the micronucleus was seldom observed. The comparison between the GSH group and the control group showed no difference （P〉0.05）. For the mice of CDDP group and GSH＋CDDP group, the bone marrow depression was distinctly, and the chromosome aberration rates and micronucleus rates were significantly increased, which were significantly different from the control group （P〈0.01）. The aberration rate in the GSH＋CDDP group was significantly lower than that in the CDDP group （P〈0.05）. These findings suggest that the glutathione has a definite protective effect on the chromosome damage induced by cisplatin.