Neuromedin B is a major bombesin-like peptide in rat brain: regional distribution of neuromedin B and neuromedin C in rat brain, pituitary and spinal cord |
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| Authors: | N Minamino K Kangawa H Matsuo |
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| Institution: | 1. Department of Biochemistry, Miyazaki Medical College, Kiyotake, Miyazaki 889-16, Japan;2. Department of Anesthesiology, Miyazaki Medical College, Kiyotake, Miyazaki 889-16, Japan |
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| Abstract: | Neuromedin B and neuromedin C are the novel mammalian bombesin-like peptides isolated from porcine spinal cord. We have developed highly specific and sensitive radioimmunoassays for neuromedin B and neuromedin C, and determined their regional distribution in rat central nervous system. Prior to measurements of the tissue contents, immunoreactive neuromedin B and C were characterized by gel-filtration and high performance liquid chromatography. Neuromedin B and C immunoreactivities have similar regional distribution in rat brain, but the content of immunoreactive neuromedin B is 2-6 times higher than that of immunoreactive neuromedin C in every region. These results indicate that neuromedin B is a major endogenous bombesin-like peptide in rat brain and has specific functions of physiological importance. |
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| Keywords: | NMB neuromedin B NMC neuromedin C NMB(O) oxidized neuromedin B NMC(O) oxidized neuromendin C RIA radioimmunoassay ir immunoreactive HPLC high performance liquid chromatography GRP gastrin releasing peptide MW molecular weight TFA trifluoroacetic acid BSA bovine serum albumin |
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