| YXXM 基序对J 亚群禽白血病病毒复制的影响 |
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| 引用本文： | 冯少珍,李娇,曹伟胜,廖明. YXXM 基序对J 亚群禽白血病病毒复制的影响[J]. 微生物学报, 2011, 51(12): 1663-1668 |
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| 作者姓名： | 冯少珍 李娇 曹伟胜 廖明 |
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| 作者单位： | 华南农业大学兽医学院,农业部动物疫病防控重点开放实验室,广州510642 |
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| 基金项目： | NSFC-广东联合基金(U0831002);国家博士后科学基金(20100470929);广东省自然科学基金(10451064201005432);广东省科技计划项目(2009A020101006);国家自然科学基金(30771612) |
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| 摘 要： | [目的]毒株NX0101是骨髓瘤病变型J亚群禽白血病病毒,其早期感染细胞能诱导PI3 K/Akt信号转导通路的激活,本文针对NX0101毒株是否存在YXXM基序及其作用进行了探讨.[方法]利用TMpred软件对NX0101毒株囊膜蛋白(Env)的氨基酸序列进行生物信息学分析,通过搭桥PCR方法将YXXM基序相应的核苷酸序列突变后,构建突变质粒并转染DF-1细胞,拯救出YXXM突变体毒株NX0101 mt( Y/F,M/A),利用real-time PCR和ELISA方法检测并比较YXXM突变前后毒株在RNA水平和蛋白水平的复制情况.[结果]NX0101毒株Env胞浆区554 -557位氨基酸存在典型的PI3K结合基序YXXM.YXXM基序突变后,病毒RNA转录水平和病毒蛋白合成水平都显著下降.[结论]YXXM基序对NX0101毒株在体外宿主细胞中复制发挥重要的作用.
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| 关 键 词： | J亚群禽白血病病毒 病毒NX0101 YXXM基序 病毒复制 |
| 收稿时间： | 2011-06-09 |
| 修稿时间： | 2011-07-10 |
Effect of YXXM motif on viral replication of subgroup J avian leukosis virus |
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| Shaozhen Feng,Jiao Li,Weisheng Cao and Ming Liao. Effect of YXXM motif on viral replication of subgroup J avian leukosis virus[J]. Acta microbiologica Sinica, 2011, 51(12): 1663-1668 |
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| Authors: | Shaozhen Feng Jiao Li Weisheng Cao Ming Liao |
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| Affiliation: | Shaozhen Feng,Jiao Li,Weisheng Cao,Ming Liao Key Laboratory of Animal Disease Control and Prevention,Ministry of Agriculture,College of Veterinary Medicine,South China Agricultural University,Guangzhou 510642,China |
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| Abstract: | [Objective] It was reported that subgroup J avian leukosis virus strain NX0101 activates PI3K/Akt pathway during early infection in DF-1 cells.Whether there is YXXM motif in the amino acid sequence of NX0101 and the function of YXXM motif were studied.[Methods] The presence of internal transmembrane domains in the envelope protein of NX0101 was analyzed by Tmpred.Point mutation was introduced to change the YXXM motif in the NX0101 strain to FXXA.The plasmid containing the full genome of NX0101 with mutation... |
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| Keywords: | subgroup J avian leukosis virus NX0101 YXXM motif virus replication |
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