Killing rate curve and combination effects of surfactin C produced from Bacillus subtilis complex BC1212 against pathogenic Mycoplasma hyopneumoniae |
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| Authors: | M H Hwang M H Kim E Gebru B Y Jung S P Lee S C Park |
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| Institution: | (1) College of Veterinary Medicine, Kyungpook National University, Daegu, 702-701, Republic of Korea;(2) Systems Microbiology Research Center, Korea Research Institute of Bioscience and Biotechnology, Daejeon, 305-806, Republic of Korea;(3) National Veterinary Research and Quarantine Service, Anyang, 430-824, Republic of Korea;(4) Department of Food Science and Technology, Keimyung University, Daegu, 704-701, Republic of Korea |
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| Abstract: | This study evaluated the killing rate as well as the antimycoplasmal effect of surfactins isolated from Bacillus subtilis complex BC1212, either alone or in combination with various antibacterials against Mycoplasma hyopneumoniae. Prior to the killing rate and the combination effect studies of surfactins, the minimal inhibitory concentrations (MICs)
of various antibacterials and surfactins (consisting of surfactins A, B, C, and D) against M. hyopneumoniae were investigated. The MIC of all surfactins was found to be 64 μg/ml. The MICs of colistin (COL), norfloxacin (NFX), oxytetracycline
(OTC), streptomycin (SM) and tiamulin (TIA) were >256, 0.063, 0.025, 4, and 0.015 μg/ml, respectively. In the killing rate
curve studies, surfactin C at 2× MIC and 4× MIC concentrations was found to reduce viability by >3 log10 c.c.u./ml within 2–4 h of incubation. Combination of surfactin C with other antibacterials showed additive interaction from
the viewpoint of fractional inhibitory concentration (FIC) index of >0.5 but ≤2 as a borderline. Taking all results into consideration,
surfactin C may be useful as a preventive or therapeutic adjuvant in the pathogenesis of mycoplasmal infection. |
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| Keywords: | Surfactin C Mycoplasma hyopneumoniae MIC FIC Killing rate curve |
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