1-Methyl-4-Phenylpyridinium Uptake by Human and Rat Striatal Synaptosomes |
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Authors: | J Willoughby R F Cowburn J A Hardy Vivette Glover M Sandler |
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Institution: | Department of Chemical Pathology, Queen Charlotte's Hospital, London, England. |
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Abstract: | 1-Methyl-4-phenylpyridinium (MPP+) was taken up into human and rat striatal synaptosomes by a saturable system, similar to that for dopamine, with Km values of 0.24 and 0.17 microM, respectively, and similar Vmax values. Uptake of MPP+ and dopamine into both rat and human synaptosomes was inhibited by cocaine and amfonelic acid, with the latter being five to 10 times more potent than the former. MPP+ uptake was potently inhibited by dopamine in preparations from both species. In general, the characteristics of human and rat synaptosomal MPP+ uptake were very similar It seems unlikely that species differences in toxicity to 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine or reaction to dopamine uptake blockers stem from this system. |
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Keywords: | 1-Methyl-4-phenyl-1 2 3 6-tetrahydropyridine 1-Methyl-4-phenylpyridinium Dopamine Human and rat striatal synaptosomes |
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