Isolation and characterization of a 2-methylphenanthrene utilizing bacterium: identification of ring cleavage metabolites |
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Authors: | J Sabaté M Grifoll M Viñas A M Solanas |
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Institution: | (1) Department of Microbiology, University of Barcelona, Diagonal 645, E-08028 Barcelona, Spain e-mail: solanas@porthos.bio.ub.es Tel.: +34-93-4021483 Fax: +34-93-4110592, ES |
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Abstract: | A bacterial strain capable of utilizing 2-methylphenanthrene (2-MP) as its sole source of carbon and energy for growth was
isolated from creosote contaminated soil. The isolate was identified as a strain of Sphingomonas sp. and was designated strain JS5. Utilization of 2-MP by strain JS5 was demonstrated by an increase in bacterial biomass
concomitant with a decrease of 2-MP in liquid mineral medium with this compound as sole source of carbon and energy. Growth
yield indicated a 23% assimilation of 2-MP carbon. Washed-cell suspensions of strain JS5 incubated with 2-MP accumulated a
major metabolite identified as 1-hydroxy-6-methyl-2-naphtoic acid, according to its UV, mass and NMR spectra, and a minor
compound with HPLC R
t
and UV spectrum indistinguishable from 5-methylsalicylate. The identification of those metabolites, and the demonstration
of 2,3-catechol dioxygenase activity in 2-MP induced cells show that the biodegradation of 2-MP by strain JS5 is initiated
via dioxygenation and meta-cleavage of the non-methylated aromatic ring, and then proceeds by reactions similar to those reported for phenanthrene.
Incubation of the strain with a MP-containing mixture from a pyrolytic fuel oil demonstrates that strain JS5 also acts on
other methylated phenanthrenes.
Received: 28 December 1998 / Received revision: 21 June 1999 / Accepted: 27 June 1999 |
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