Cellular distribution of RNA populations in 16-cell stage embryos of the sand dollar, Dendraster excentricus |
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Authors: | S Mizuno Y R Lee A H Whiteley H R Whiteley |
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Institution: | 1. Department of Microbiology, University of Washington, Seattle, WashingtonUSA;2. Department of Zoology, University of Washington, Seattle, WashingtonUSA;3. Friday Harbor Laboratories, University of Washington, Seattle, WashingtonUSA |
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Abstract: | RNA was extracted from pure preparations of micromeres and meso-plus macromeres isolated from 16-cell stage embryos of Dendraster excentricus. Molecular hybridization-competition experiments disclosed that the binding of 16-cell stage labeled RNA to denatured sperm DNA was competed equally well by micromere RNA, meso-plus macromere RNA, total 16-cell RNA and unfertilized egg RNA, indicating the egg-type populations were distributed almost equally in the different blastomeres. In contrast, experiments with 3H-RNA extracted from micromeres obtained from pulse-labeled 16-cell stage embryos showed qualitative differences when unfertilized egg RNA and total 16-cell stage RNA were used as competitors. Such differences in RNA populations could not be detected in 3H-RNA isolated from the meso-plus macromere fraction. |
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