Fast potentiation of glycine receptor channels of intracellular calcium in neurons and transfected cells |
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Authors: | Fucile S De Saint Jan D de Carvalho L P Bregestovski P |
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Institution: | Laboratoire de Biologie Cellulaire et Moléculaire du Neurone, Institut Pasteur, Paris, France. |
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Abstract: | Inhibitory glycine receptors (GlyRs) are mainly expressed in the spinal cord and in the midbrain, where they control motor and sensory pathways. We describe here a fast potentiation of GlyR by intracellular Ca2+. This phenomenon was observed in rat spinal cord neurons and in transfected human cell lines. Potentiation develops in <100 ms, is proportional to Ca2+ influx, and is characterized by an increase in GlyR apparent affinity for glycine. Phosphorylation and G protein pathways appear not to be involved in the potentiation mechanism. Single-channel recordings in cell-attached and excised patches, as well as whole-cell data suggest the presence of a diffusible cytoplasmic factor that modulates the GlyR channel gating properties. Ca2+-induced potentiation may be important for rapid modulation of glycinergic synapses. |
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