Evidence for dominant suppression of repeat-induced point mutation (RIP) in crosses with the wild-isolatedNeurospora crassa strains Sugartown and Adiopodoume-7 |
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Authors: | Felicite K Noubissi K Aparna Kevin McCluskey Durgadas P Kasbekar |
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Institution: | (1) Centre for Cellular and Molecular Biology, 500 007 Hyderabad, India;(2) Fungal Genetics Stock Center, Department of Microbiology, University of Kansas Medical Center, KS 66103 Kansas City, USA |
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Abstract: | A convenient assay to score repeat-induced point mutation (RIP) inNeurospora employs theerg-3 locus as a mutagenesis target. Using this assay we screened 132 wild-isolatedNeurospora crassa strains for ability to dominantly suppress RIP. RIP was exceptionally inefficient in crosses with the wild isolates Sugartown
(P0854) and Adiopodoume-7 (P4305), thereby suggesting the presence of dominant RIP suppressors in these strains. In other
experiments, we found no evidence for dominant RIP suppression by theSpore killer haplotypesSk-2 andSk-3. |
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Keywords: | Spore killer meiotic drive Neurospora crassa RIP |
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