Isolation and characterization of four polycyclic aromatic hydrocarbon degrading bacteria from soil near an oil refinery |
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Authors: | BT Ashok S Saxena J Musarrat |
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Institution: | Interdisciplinary Biotechnology Unit, AMU, Aligarh, Uttar Pradesh, India |
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Abstract: | Four bacterial strains (I-IV) capable of optimum growth on 0·1% naphthalene, anthracene or a mixture of naphthalene and phenanthrene were isolated from soil near an oil refinery. Two isolates (I and II) were identified as belonging to the genus Micrococcus , while strains III and IV were identified as Pseudomonas and Atcaligenes respectively. All the isolates were found to bear high molecular weight plasmid DNA (isolate I and IV 89%, II 67·5% and III 92·1% of Λ DNA), which is presumed to aid in the metabolism of polycyclic aromatic hydrocarbons. The strains also showed appreciable growth at high concentrations of NaCl (up to 7·5%). |
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