Comparison of nonhistone chromosomal proteins from neuronal and glial chromatin by isoelectric focussing and microdisc electrophoresis. |
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| Authors: | H Fleischer-Lambropoulos K Pollow |
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| Institution: | Institut für Molekularbiologie und Biochemie, Freie Universität Berlin, F.R.G. |
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| Abstract: | The present study describes optimal conditions for preparative fractionation of nonhistone chromosomal proteins from neuronal and glial nuclei.We have detected about 1200 nonhistone chromosomal proteins. Microgels of glial origin contained 20% more protein bands than those of neuronal origin. However, neuronal bands prevailed clearly in the acidic respectively in the high-molecular range. Amino acid analysis confirmed the observed heterogeneity. |
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