Physical analysis of in vivo pCI301 fusion plasmids in Lactococcus lactis subsp. lactis |
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| Authors: | Finbarr Hayes Charles Daly Gerald F Fitzgerald |
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| Institution: | Department of Food Microbiology and National Food Biotechnology Centre, University College, Cork, Ireland |
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| Abstract: | Abstract In vivo fusion plasmids identified following conjugative mobilization of pCI301, the 75-kilobase (kb) lactose-proteinase plasmid of Lactococcus lactis subsp. lactis UC317, were characterized. These plasmids (95 kb) were generated from fusion-deletion events involving pCI301 and the 38-kb UC317-derived cryptic plasmid, pCI303. Recombinant plasmids were separable into distinct classes based on their associated phenotypes and restriction maps. The formation of pCI301: : pCI303 composite plasmids within strain UC317 was also demonstrated. |
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| Keywords: | Lactococcus lactis subsp lactis Plasmid recombination Plasmid deletion Conjugation pCI301 |
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