Reduction of RNA and DNA in Methylococcus capsulatus by endogenous nucleases |
| |
Authors: | J Larsen L Joergensen |
| |
Institution: | (1) DB Lab, Dansk Bioprotein A/S, Stenhuggervej 9, 5230 Odense M, Denmark. Fax:+45 65 93 23 12, DK |
| |
Abstract: | A method for reducing RNA and DNA in the bacterium Methylococcus capsulatus (Bath) has been developed. Endogenous RNase and DNase were activated by a 10 s heat shock at 90°C. Cells were then incubated
at 60°C for 20 min to allow degradation of the nucleic acids. The optimum pH for the process was 7.0. The protein loss was
less than 10% and occurred during the initial heat shock. No protein loss was found during incubation. The total dry-weight
loss in connection with an 80% reduction of the nucleic acid content was 20%–25%, giving a final product with a raw protein
content of approximately 75%. Reduction of both RNA and DNA was inhibited by CuSO4 and ZnSO4. DNA reduction was stimulated by other minerals. Optimal stimulation was found at 1 mM FeSO4. Reduction of RNA was not increased by any of the minerals tested.
Received: 29 June 1995/Received last revision: 2 October 1995/Accepted: 16 October 1995 |
| |
Keywords: | |
本文献已被 SpringerLink 等数据库收录! |
|