Initiation of polyribosome formation in poliovirus-infected HeLa cells |
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| Authors: | A S Huang D Balitmore |
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| Affiliation: | 1. Institute for Molecular Engineering, University of Chicago, Chicago, IL 60637, USA;2. Department of Pediatrics, Section of Hematology/Oncology, University of Chicago, Chicago, IL 60637, USA;3. Institute for Molecular Engineering, Argonne National Laboratory, Argonne, IL 60439, USA;4. Department of Biomedical Engineering, University of Southern California, Los Angeles, CA 90089, USA;5. Medical Scientist Training Program, University of Chicago, Chicago, IL 60637, USA;6. Biophysical Sciences Graduate Program, University of Chicago, Chicago, IL 60637, USA |
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| Abstract: | Poliovirus RNA is synthesized in the replication complex and is released free of ribosomes. Much of the RNA then goes on to become part of polyribosomes, a process requiring at least ten minutes. As isolated in low ionic strength buffer, the viral RNA recovered from polyribosomes is coated with protein and has a buoyant density in CsCl of 1.40. Dissociation of polyribosomes with puromycin leads to two predominant complexes banding at buoyant densities of 1.44 and 1.47. Similar complexes are formed by viral RNA synthesized in the presence of cycloheximide and can be shown to be precursors of polyribosomes. |
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