Sequential mechanism of refolding of carbonic anhydrase B |
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Authors: | G V Semisotnov N A Rodionova V P Kutyshenko B Ebert J Blanck O B Ptitsyn |
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Institution: | Institute of Protein Research, Academy of Sciences of the USSR, Pushchino, Moscow Region. |
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Abstract: | The kinetics of refolding of bovine carbonic anhydrase B was studied by a variety of methods over a wide range of times (from milliseconds to hours). It has been shown that protein refolding proceeds through three stages. At the first stage (t1/2 approximately equal to 0.03 s) hydrophobic clusters and a compact state of the chain are formed. At the second stage (t1/2 approximately equal to 140 s) hydrophobic clusters are desolvated and the rigid native-like hydrophobic core is formed. At the third stage (t1/2 approximately equal to 600 s) the native active protein is formed. |
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