Analysis of chloroplast and mitochondrial DNA in asymmetric somatic hybrids betweenSolanum tuberosum and irradiatedS. brevidens |
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Authors: | Yong-Sheng Xu Eija Pehu |
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Institution: | (1) Department of Plant Production, University of Helsinki, Box 27, SF-00014, Finland;(2) Coriell Institute for Medical Research, 401 Haddon Avenue, 08103 Camden, NJ, USA |
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Abstract: | Organellar DNA of asymmetric somatic hybrids betweenSolanum tuberosum and irradiatedS. brevidens were analysed by DNA hybridization methods using the spinach chloroplast probepSBD, wheat mitochondrial genenad5 and petunia mitochondrial geneorf25. Eight of the 12 asymmetric hybrid plants hadS. tuberosum chloroplast DNA and the remaining fourS. brevidens chloroplast DNA. A novel mitochondrial hybridization pattern was present in eight out of the 17 hybrids tested. In six hybrids,
novel combinations of chloroplasts and mitochondria were found, indicating that both organelle types sorted out independently. |
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Keywords: | asymmetric somatic hybrids organellar DNA Solanum tuberosum Solanum brevidens |
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