HDA19调控拟南芥对真菌Botrytis cineara的抗性反应

HDACs(Histone deacetylase)家族蛋白质负责组蛋白H3K4和H4K19脱乙酰化,并参与植物生长和应激反应的信号转导过程。茉莉酮酸酯Jasmonates(JA)是一种重要的天然植物激素,不仅调节植物生长和发育而且还参与植物对多种逆境胁迫响应的信号转导和调控过程。但是,HDACs在植物中参与JA信号转导的具体机制目前还不是很清楚。该研究以HDA19(Histone deacetylase 19)为对象,探讨了HDACs在植物JA信号转导中的功能和作用。结果表明:HDA19的T-DNA插入纯合突变体在JA处理条件下没有出现明显的JA根长反应。在相同处理条件下,hda19的不同突变体株系与相同生态型背景的野生型植株(WT)花色素苷含量无显著差异,但下游JAZ1、VSP1等JA信号通路的标记基因都显著上调表达。同时,hda19相比于WT对真菌Botrytis cineara的抗性显著增强,且hda19中下游基础防御标记基因PDF1.2、Thi2.1、ERF1等的表达水平显著高于WT。基于上述研究结果,该研究认为HAD19通过JA信号通路负调控拟南芥对真菌B.cineara的防御反应。

Regulation of HDA19 on resistance of Arabidopsis thaliana to Botrytis cineara

HDACs(Histone deacetylase)family proteins are responsible for the deacetylation of histones H3K4 and H4K19 and are involved in signal transduction processes of plant growth and stress. Jasmonates(JA)is an important natural plant hormone that not only regulates plant growth and development but also participates in the process of signal transduction and regulation of plant responses to multiple stress conditions. However, the specific mechanism by which HDACs participate in JA signal transduction is not very clear. We investigated the function and role of HDACs in plant JA signal transduction with HDA19(Histone deacetylase 19). In this study, we found that the HDA19 T-DNA insertion homozygous mutant did not show a significant JA root length response treated by MeJA. Under the same conditions, there was no significant difference in anthocyanin content between different mutant lines of hda19 and WT plants of the same ecotype background. However, JAZ1, VSP1 and other downstream JA signaling pathway marker genes were significantly up-regulated expression. At the same time, the resistance of hda19 to fungi Botrytis cineara was significantly enhanced compared with WT, and the expression levels of basic defense marker genes PDF1.2, Thi2.1 and ERF1 were signifi-cantly higher than WT in hda19. Based on these results, we believe that HAD19 negatively regulates Arabidopsis thaliana defense against Botrytis cineara via JA signaling pathway.