白花泡桐优树组织培养及产业化快繁技术

以自选育的白花泡桐优树茎段为外植体,进行种苗组培快繁技术研究。结果表明:其最佳的外植体灭菌方法是以0.1%升汞处理7 min;合适的初代诱导培养基为MS+6-BA 2.0 mg·L~(-1)+IBA 0.2 mg·L~(-1)+糖30 g·L~(-1)+琼脂3.5 g·L~(-1)(pH 5.8),培养30 d,芽诱导率70%;合适的继代增殖方法为在高浓度植物生长物质培养基MS+6-BA 4.0 mg·L~(-1)+IBA 0.4 mg·L~(-1)+蔗糖30 g·L~(-1)+琼脂3.5 g·L~(-1)(pH 5.8)和低浓度植物生长物质培养基MS+6-BA 0.4 mg·L~(-1)+IBA 0.04 mg·L~(-1)+蔗糖30 g·L~(-1)+琼脂3.5 g·L~(-1)(pH 5.8)中交替培养,获得的丛生芽长势良好,玻璃化率低于5%,增殖系数大于6.0/25 d;最适的生根培养基为1/2MS+NAA0.2 mg·L~(-1)+蔗糖20 g·L~(-1)+卡拉胶3.4 g·L~(-1)(pH 5.8),培养14 d,得到白花泡桐生根苗,每株长根5~10条,根长3~5 cm,生根率98%,根系洁白、根毛少而短,易于清洗。将生根苗按照常规方法炼苗后移栽于温室大棚中,50 d后即可出圃,此时平均苗高1.0 m、地径1.0~2.0 cm,成活率在90%以上。

Tissue culture and rapid propagation technique of the superior tree from Paulownia fortunei

Using excellent stem segments of Paulownia fortunei as explants, tissue culture and rapid propagation technique for P. fortunei were studied in this paper. The results showed that the optimal sterilization time of 0.1% HgCl₂ was 7 m; the suitable primary induction medium was MS+ 6-BA 2.0 mg·L^-1 + IBA 0.2 mg·L^-1+ sucrose 30 g·L^-1+ agar 3.5 g·L^-1(pH 5.8), 30 d later, bud induction rate was 70%; the suitable method for multiplication culture was MS+ 6-BA 4.0 mg·L^-1+ IBA 0.4 mg·L^-1+ sucrose 30 g·L^-1+ agar 3.5 g·L^-1(pH 5.8)and MS+ 6-BA 0.4 mg·L^-1+ IBA 0.04 mg·L^-1+ sucrose 30 g·L^-1+ agar 3.5 g·L^-1(pH 5.8)alternately,health and vitrification multiple shoots with this method was lower than 5%, multiplication factor was higher than 6.0/25 d; the optimal medium for rooting was 1/2MS + NAA 0.2 mg·L^-1+ sucrose 20 g·L^-1+ carrageenan 3.4 g·L^-1(pH 5.8), 14 d later, the rooting seedlings were obtained, rooting number were 5-10, root length was 3-5 cm, the rooting rate was 98% and whose roots were white, with less and short hair, easy to clean. Through acclimatization, the rooting seedlings were transplanted in the greenhouse, 50 d later, whose average height was 1.0 m, ground diameter was 1.0-2.0 cm, and survival rate was above 90%.