木奈WRKY同源基因的分离与表达分析

以木奈（Prunus salicina Lindli.var cordata J.Y.Zhang et al.）为试验材料，采用稀释池PCR法，筛选木奈叶片全长均一化cDNA文库，获得了木奈WRKY同源基因的12个全长cDNA分别命名为PsWRKY7、PsWRKY14、PsWRKY20、PsWRKY21、PsWRKY22、PsWRKY29、PsWRKY31、PsWRKY32、PsWRKY33、PsWRKY40、PsWRKY46和PsWRKY75，长度分别为1 517、1 798、2 098、1 177、1 242、1 396、2 135、1 760、2 113、1 047、1 258和700 bp，ORF长度分别为1 095、1 572、1 764、1 068、1 071、1 002、1 944、1 602、1 770、963、1 077和672 bp，分别编码364、523、587、355、357、333、647、533、589、320、358、223个氨基酸。荧光定量结果显示，12个WRKY同源基因在1.0 mmol/L水杨酸处理后，所获得的PsWRKY表达量均显著上调，表明木奈WRKY基因可能是水杨酸诱导防御反应的重要调控因子。

Isolation and Expression of WRKY Homologous Genes from Prunus salicina var. cordata

In this study, Prunus salicina Lindli. var. cordata J.Y.Zhang et al was chosen as experiment material, the dilution pool PCR method was used to screen a normalized full length cDNA library of P. salicina var. cordata leaves and to obtain 12 the full length cDNA of WRKY homologous genes, which are named PsWRKY7, PsWRKY14, PsWRKY20, PsWRKY21, PsWRKY22, PsWRKY29, PsWRKY31, PsWRKY32, PsWRKY33, PsWRKY40, PsWRKY46 and PsWRKY75, respectively. Their length are 1 517, 1 798, 2 098, 1 177, 1 242, 1 396, 2 135, 1 760, 2 113, 1 047, 1 258 and 700 bp, respectively. Their ORF lengths are respective 11 095, 1 572, 1 764, 1 068, 1 071, 1 002, 1 944, 1 602, 1 770, 963, 1 077 and 672 bp. They are encoded as 364, 523, 587, 355, 357, 333, 647, 533, 589, 320, 358 and 223 amino acids, respectively. The results of Real Time PCR showed that PsWRKY expression levels increased significantly after spraying with 1.0mmol/L salicylic acid. This result indicated that PsWRKY might be important regulatory factor for inducing plant defense response by salicylic acid.