The distribution of sulfur among various components of the wheat grain

Summary 1. Radioactive sulfur (S³⁵) was introduced into 3 sets of wheat plants grown hydroponically in the greenhouse. When mature, the grain was milled using micromilling procedures.2. Because of the small quantity of samples obtained, a method was developed for concentrating small amounts of sulfate.3. The highest concentration of S³⁵ in the mechanically separated fractions was in the germ, with bran, flour (endosperm), shorts, and red dog containing lesser amounts in that order.4. Radioactivity was approximately the same in the total water-soluble and gluten fractions of flour, although the percentage of nitrogen of total water-solubles was less than gluten.5. Only small differences in count rate were found between the gluten and the water-soluble proteins of the flour, indicating that much of the sulfur radioactivity in the total water-solubles was due to material other than protein.6. S³⁵ in the crude starch was almost completely in the tailings fraction.7. Gliadin was compared with glutenin and found to be somewhat higher in count rate, although nitrogen percentages of both were approximately the same.Contribution No. 538, Department of Botany and Plant Pathology; Kansas Agricultural Experiment Station, Kansas State University, Manhattan. Botany Department serial No. 694.Portion of a dissertation presented as partial fulfillment of the requirement for the Doctor of Philosophy degree in the Department of Botany and Plant Pathology, Kansas State University.