Processing stored stallion semen doses by Single Layer Centrifugation |
| |
| Authors: | Morrell J M Garcia B Macias Pena F J Johannisson A |
| |
| Institution: | aClinical Sciences, Swedish University of Agricultural Sciences (SLU), Uppsala, Sweden;bUniversity of Extremadura, Caceres, Spain;cDepartment of Anatomy, Physiology and Biochemistry, SLU, Uppsala, Sweden |
| |
| Abstract: | The purpose of this study was to determine if the quality of stored stallion semen doses could be enhanced by the scaled-up version of Single Layer Centrifugation using Androcoll-E-Large. Three semen doses from each of fifteen stallions were transported overnight to the Swedish University of Agricultural Sciences (SLU) for processing 24 h after semen collection. Sperm quality in the resulting SLC-selected samples was significantly improved compared to the uncentrifuged samples: mean progressive motility was increased by 8% on the day of processing (P < 0.001) and by 13% after 24 h cold storage (P < 0.001), normal morphology was increased by 4% (P < 0.01), whereas mean %DFI was decreased by 2% (P < 0.001). When these SLC-selected samples were compared retrospectively to fresh samples processed by SLC with Androcoll-E Small, sperm quality was found to be similar, although it was not maintained for as long in the sperm samples stored before SLC. These results suggest an additional option for improving sperm quality in stallion semen doses for artificial insemination. |
| |
| Keywords: | Single Layer Centrifugation Stored equine semen Androcoll-E |
| 本文献已被 ScienceDirect PubMed 等数据库收录！ |
|
