Degradation of chlorotoluenes by in vivo constructed hybrid strains: problems of enzyme specificity, induction and prevention of meta-pathway |
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Authors: | Ulrich Brinkmann Walter Reineke |
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Affiliation: | Bergische Universit?t-Gesamthochschule Wuppertal, Chemische Mikrobiologie, FRG. |
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Abstract: | The activities of the TOL plasmid-coded xylene oxygenase, benzylalcohol dehydrogenase, benzaldehyde dehydrogenase of Pseudomonas putida strain PaW1 were tested with substituted toluenes, benzylalcohols and benzaldehydes, respectively, as substrates. Several chlorinated toluenes were shown to induce enzymes of the xylene degradation sequence. Conjugative transfer of the TOL plasmid from Pseudomonas putida strain PaW1 to Pseudomonas sp. strain B13 and Pseudomonas cepacia strain JH230 allowed the isolation of hybrid strains capable of growing in the presence of 3-chloro-, 4-chloro- and 3,5-dichlorotoluene. Hybrid strains revealed new ways to prevent the dead-end meta-pathway for cholorocatechols. |
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Keywords: | Chlorotoluenes Pseudomonas putida strain PaW1 Pseudomonas sp. strain B13 Pseudomonas cepacia strain JH230 Hybrid strain Strain construction Enzyme specificity |
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