Improving signal intensities for genes with low-expression on oligonucleotide microarrays |
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| Authors: | Latha Ramdas David E Cogdell Jack Y Jia Ellen E Taylor Valerie R Dunmire Limei Hu Stanley R Hamilton Wei Zhang |
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| Institution: | (1) Cancer Genomics Core Laboratory, Department of Pathology, The University of Texas M. D. Anderson Cancer Center Houston, 77030 Houston, TX, USA |
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| Abstract: | Background DNA microarrays using long oligonucleotide probes are widely used to evaluate gene expression in biological samples. These
oligonucleotides are pre-synthesized and sequence-optimized to represent specific genes with minimal cross-hybridization to
homologous genes. Probe length and concentration are critical factors for signal sensitivity, particularly when genes with
various expression levels are being tested. We evaluated the effects of oligonucleotide probe length and concentration on
signal intensity measurements of the expression levels of genes in a target sample. |
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