细胞质雄性不育水稻线粒体基因组的RFLP分析

利用RFLP技术，比较研究了在农业生产上广泛应用的9种细胞质雄性不育体系的线粒体基因组，结果表明：1）9种水稻雄性不育细胞质的遗传相似性变化范围为0．615～1．000。所有配子体雄性不育细胞质的遗传相似性变化范围为0．6431．000，其中QXA，DY1A和YM15A这3种细胞质的遗传相似性为1．000。所有孢子体雄性不育细胞质的遗传相似性为1．000,2）在配子体细胞质雄性不育水稻中，用3个探针（atpa，atp9＋nad6，cox2）与2种内切酶（HindⅢ，BamHⅠ）的组合未发现不育系与保持系之间的多态性，但在探针atp6，cob，和had2的杂交带型中找到了不育系和保持系之间的一些差异。YTA和YTB在5个探针．内切酶组合（atp61HindⅢ，coblHindⅢ，atp61BamHⅠ，coblBamHⅠ，nad21BamHⅠ）中存在差异。3种细胞质（QX，SJ，DY1）的不育系和保持系之间的差异是相同的，都出现在atp6／HindⅢ，atp6／BamHⅠ，cob／BamHⅠ等3个组合中。YM15A和YM15B在4个组合（atp6／HindⅢ，atp6／BamHⅠ，cob／BamHⅠ，nad2／BamHⅠ）中存在差异。LYA和LYB的差异出现在cob／HindⅢ，cob／BamHⅠ，nad2／BamHⅠ这3个组合中；3）在孢子体细胞质雄性不育水稻中，所有不育系的带型是一样的，所有保持系的带型也一样。不育系和保持系的差异出现在atp6／HindⅢ，cob／HindⅢ，atp6／EcoR，Ⅰcob／EcoRⅠ，cox1／EcoRⅠ，atp6／BamHⅠ，cob／BamHⅠ，cox1／BamHⅠ，cox2／BamHⅠ等9个组合中。这些结果在分子水平上揭示了9种雄性不育细胞质的线粒体基因组存在结构多样性，并为其细胞质雄性不育分子机理的研究打下了基础。

RFLP Analysis for Mitochondrial Genome of CMS-Rice

Restriction fragment length polymorphism (RFLP) was used to analyze mitochondrial (mt) genome of cytoplasmic male sterility (CMS) rice. Differences were observed among mitochondrial genomes of the sterile line (A) and maintain line (B) of nine types of CMS rice; Mitochondrial genomic differences were also detected between A and B in many functional gene regions. Even the materials with the same nucleic background have differences in their mtDNA. This provides molecular evidence for the cyto-plasmic heterogeneity and the CMS mechanism research.