The binding of monosaccharides to wheat germ agglutinin: fluorescence and NMR investigations |
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Authors: | P Midoux J P Grivet F Delmotte M Monsigny |
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Affiliation: | Centre de Biophysique Moléculaire, C.N.R.S., 45045 Orleans, France, and Université d''Orléans, 45046 Orleans, France |
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Abstract: | The synthesis of N-acetyl- and N-trifluoroacetyl-glucosaminides was reported. The interaction of these compounds with wheat germ agglutinin, a plant lectin specific for N-acetyl-glucosamine and sialic acid, was investigated by two complementary approaches: 1H and 19F NMR, and fluorescence spectroscopy. This last technique relies on the existence of a competitive equilibrium involving the protein, the ligand and O-(methylumbelliferyl)-N-acetyl-glucosaminide, a fluorescent saccharide. The binding constants and the chemical shifts in the complex were determined and were related to the protein structure. |
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Keywords: | WGA wheat germ agglutinin αMeGlcNTFA βMeGlcNTFA βMeGlcNAc βMUFGlcNAc 6-deoxyGlcNAc 2,6-dideoxy-2-acetamido-D-glucose DSS sodium 2,2-dimethyl-2-silapentane-5-sulfonate |
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