Agrobacterium rhizogenes-mediated transformation of Echinacea purpurea |
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| Authors: | M Trypsteen M Van Lijsebettens R Van Severen M Van Montagu |
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| Institution: | (1) Laboratory of Pharmacognosy and Phytochemistry, State University of Ghent, Harelbekestraat 72, B-9000 Gent, Belgium;(2) Laboratory of Genetics, State University of Ghent, Ledeganckstraat 35, B-9000 Gent, Belgium |
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| Abstract: | Summary
Echinacea purpurea seedlings were inoculated with several Agrobacterium rhizogenes strains in order to obtain hairy roots. Infection with A. rhizogenes strains LMG63 and LMG150 resulted in callus formation. Upon infection with strains ATCC 15834 and R1601 hairy roots were obtained. Opine detection confirmed transformation of E. purpurea. Comparative HPLC fingerprint analysis of the alkamides from natural plant source, control tissues, and transformed callus and roots indicated that transformed callus and hairy roots might be a promising source for continuous and standardized production of the dodeca-2E,4E,8Z,10E/Z-tetraenoic acid isobutylamide and related amides.Abbreviations HPLC
high-pressure liquid chromatography
- MS
Murashige and Skoog culture medium |
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| Keywords: | Agrobacterium rhizogenes alkamides Echinacea purpureal transformation |
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