Cloning and expression of the genes for ribulose-1,5-bisphosphate carboxylase from Spirulina platensis |
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Authors: | Orsola Tiboni Giuseppe Di Pasquale Orio Ciferri |
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Affiliation: | Department of Genetics and Microbiology ‘A. Buzzati Traverso’, University of Pavia, 27100 Pavia Italy |
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Abstract: | The genes for the large and small subunits of ribulose-1,5-bisphosphate carboxylase have been cloned from the filamentous cyanobacterium Spirulina platensis. The two genes, located very closely on a 4.6 kbp DNA fragment, appear to be expressed although to a different extent in minicells of Escherichia coli. The amount of large subunit produced in the bacterial host represents at least 10% of the total protein. |
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Keywords: | Ribulose-1,5-bisphosphate carboxylase Gene expression Cloning (S. platensis) |
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