| 水稻蜡质基因5'非翻译区一个与调控有关的内含子 |
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| 引用本文： | 蔡秀玲,王宗阳,郑霏琴,洪孟民.水稻蜡质基因5'非翻译区一个与调控有关的内含子[J].植物生理与分子生物学学报,1997(3). |
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| 作者姓名： | 蔡秀玲 王宗阳 郑霏琴 洪孟民 |
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| 作者单位： | 中国科学院上海植物生理研究所!上海，200032，中国科学院上海植物生理研究所!上海，200032，中国科学院上海植物生理研究所!上海，200032，中国科学院上海植物生理研究所!上海，200032 |
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| 摘 要： | 从发育的水稻种子中分离出RNA，经RT－PCR反应并结合顺序测定，在籼稻232蜡质基因编码区5′上游非翻译区中证明确实存在一个长度为1126bp的内含于，其A＋T碱基的含量高达67．4％，它的边界符合真核基因内含子的GT-AG规则。表明该内含子与蜡质基因的表达调控有一定的关系。
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| 关 键 词： | 第1内含子 蜡质基因 水稻 |
A Regulation-related Intron in 5' Untranslated Region of Rice Waxy Gene |
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| CAI Xiu-Ling, WANG Zong-Yang, ZHENG Fei-Qin and HONG Meng-Min.A Regulation-related Intron in 5' Untranslated Region of Rice Waxy Gene[J].Journal Of Plant Physiology and Molecular Biology,1997(3). |
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| Authors: | CAI Xiu-Ling WANG Zong-Yang ZHENG Fei-Qin and HONG Meng-Min |
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| Abstract: | Using the methods of polymerasechain reaction-amplified reverse-transcribed RNA isolated from the developing seeds and sequence analysis of amplified product. an intron of 11 26 bpin size was identified in the 5' -untranslated region of rice Oryza satira subsp.indica 232 cultivar Wx gene (Figs 2,3). Its A T content was found to be67. 4% (Table 1). The border sequences of this nitron conform to theGT-AG rule. Considering similar results ot previous experiments, it is suggested that this nitron may play an important role in the regulation of Wxgene expression. |
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| Keywords: | nitron 1 Wx gene rice |
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