| 林麝及马麝SRY基因片段克隆及其在系统进化分析中的应用 |
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| 引用本文： | 张亮,邹方东,陈三,赵尔宓,岳碧松.林麝及马麝SRY基因片段克隆及其在系统进化分析中的应用[J].动物学研究,2004,25(4):334-340. |
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| 作者姓名： | 张亮 邹方东 陈三 赵尔宓 岳碧松 |
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| 作者单位： | 1. 四川大学,生命科学学院,四川,成都,610064
2. 四川养麝研究所,四川,成都,611830 |
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| 基金项目： | 国家科技部重大基础研究前期研究专项资助项目 ( 2 0 0 3CCA0 3 0 0 0 ) |
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| 摘 要： | 根据已报道的偶蹄目动物并参照人和狗SRY基因碱基序列设计一对简并引物，以林麝(Moschus berezovskii)及马麝(M．chrysogaster)基因组DNA为模板，通过PCR扩增出SRY基因CDS区。克隆的林麝及马麝SRY基因片段经测序，其长度为684bp。从父亲遗传角度出发，利用GenBank中收录的18种偶蹄目SRY基因，用邻位相接法(NJ)和最大简约法(MP)分别构建了鹿科、牛科和麝科的系统进化树。聚类树显示麝形成一个单系，支持麝作为独立一科的观点。
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| 关 键 词： | 林麝 马麝 SRY 基因片段 克隆 系统进化 父系遗传 |
| 文章编号： | 0254-5853(2004)04-0334-07 |
Cloning of SRY Gene from Moschus berezovskii and M.chrysogaster and Its Application in Phylogenetic Analysis |
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| ZHANG Liang,ZOU Fang-dong,CHEN San,ZHAO Er-mi,YUE Bi-song.Cloning of SRY Gene from Moschus berezovskii and M.chrysogaster and Its Application in Phylogenetic Analysis[J].Zoological Research,2004,25(4):334-340. |
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| Authors: | ZHANG Liang ZOU Fang-dong CHEN San ZHAO Er-mi YUE Bi-song |
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| Institution: | ZHANG Liang~1,ZOU Fang-dong~1,CHEN San~2,ZHAO Er-mi~1,YUE Bi-song~ |
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| Abstract: | A pair of degenerate primers was designed according to the reported SRY sequences about human, dog and Artiodactyla animals.Two 684 bp CDS regions of SRY gene were cloned and sequenced from male Moschus berezovskii and male M.chrysogaster genomic DNA by PCR method.Sequence analysis were conducted in the light of paternal inheritance and the SRY sequences from M.berezovskii,M.chrysogaster,and the other 18 Artiodactyla animals were used for phylogenetic tree construction by using neighbor-joining (NJ) analysis of MEGA2.1 and maximum parsimony method (MP) analysis of PAUP4.0.The phylogeny showed that the musk deer formed a monophyletic group and it supported that the musk deer should be classified as an individual family Moschidae,instead of a subfamily or a genus. |
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| Keywords: | Moschus berezovskii Moschus chrysogaster SRY Phylogeny Paternal inheritance |
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