Cytotoxin-converting phages, φCTX and PS21, are R pyocin-related phages |
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| Authors: | Tetsuya Hayashi Hideki Matsumoto Makoto Ohnishi Shin-ichi Yokota Tomoyuki Shinomiya Makoto Kageyama Yoshiro Terawaki |
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| Affiliation: | Department of Bacteriology, Shinshu University School of Medicine, Asahi 3-1-1, Matsumoto 390, Japan; Sumitomo Pharmaceutical Research Center, Konohana-Ku, Osaka 554, Japan; Mitsubishi Kasei Institute of Life Sciences, Minamiooya, Machida, 194, Japan |
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| Abstract: | Abstract φCTX is a temperate phage of Pseudomonas aeruginosa harbouring the ctx gene that encodes cytotoxin (CTX). We identified φCTX as an R pyocin-related phage, by serological and molecular analysis, based on the findings that the infectivity of the phage was inhibited with the antisera directed R pyocins and R pyocin-related phages and that the φCTX genome showed DNA homology to the genome of PS17 (a representative of the R pyocin-related phages) as well as to the pyocin R2 genes. Another new CTX-converting, R pyocin-related phage named PS21 was isolated from a CTX-producing strain of P. aeruginosa , suggesting the distribution of the ctx gene by certain members of R pyocin-related phage family. |
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| Keywords: | Pseudomonas aeruginosa Phage Cytotoxin Phage conversion R pyocin |
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