Changes in DNA-marker frequencies associated with response to contrasting selection methods in Arabidopsis |
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Authors: | G Sills J Nienhuis |
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Institution: | (1) Sweetbriar Development Inc., 1767 San Juan Road, Watsonville, CA 95076, USA Fax: +1 408-722-0191, US;(2) Department of Horticulture, University of Wisconsin, Madison, WI 53706, XX |
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Abstract: | Recurrent selection for specific combining ability (RS-SCA) and S1 family performance (RS-S1) were compared in replicated
selection programs initiated from a common C0 base population of Arabidopsis. Three cycles of selection for aerial biomass were completed in each of two replicate programs of each selection method.
Response to selection was measured on the basis of per se, S1 progeny, and testcross performance with a common tester. All
selection programs improved testcross performance. Testcross gain per cycle in RS-S1 (7.15% cycle-1) and RS-SCA (5.31% cycle-1) were not significantly different. Performance of S1 progeny and populations per se significantly improved over cycles of
selection using RS-S1 but were unchanged by RS-SCA. Codominant molecular marker-allele frequencies were recorded for each
population at 22 polymorphic loci. Trends in marker-allele frequencies were tested by linear regression. Significant trends
in marker-allele frequencies pooled over replicate programs were detected at 8 and 7 loci in the RS-S1 and RS-SCA programs,
respectively. Marker alleles at 2 loci significantly changed frequency in response to both RS-S1 and RS-SCA programs. Marker
alleles at 6 loci significantly changed frequency only in response to RS-SCA. Marker alleles at 6 other loci showed significant
linear trends pooled over replicates only in RS-S1. No markers revealed increases in the frequency of different marker alleles
within loci using the two selection methods. Possible genetic causes of marker frequency changes are discussed, as well as
breeding implications. |
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Keywords: | Gene frequency Recurrent selection Specific combining ability |
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