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Conserved abundance and topological features in chromatin‐remodeling protein interaction networks |
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| Authors: | Damir Herman Sreenivasa R Ramisetty Yong Cai Jingji Jin Ronald C Conaway Joan W Conaway Laurence Florens Michael P Washburn |
| Institution: | 1. Ayasdi, Inc., Palo Alto, CA, USA;2. Idexx Laboratories, Westbrook, ME, USA;3. College of Life Sciences, Jilin University, Changchun, China;4. Stowers Institute for Medical Research, Kansas City, MO, USA;5. Department of Biochemistry and Molecular Biology, The University of Kansas Medical Center, Kansas City, MO, USA;6. Department of Pathology and Laboratory Medicine, The University of Kansas Medical Center, Kansas City, MO, USA |
| Abstract: | The study of conserved protein interaction networks seeks to better understand the evolution and regulation of protein interactions. Here, we present a quantitative proteomic analysis of 18 orthologous baits from three distinct chromatin‐remodeling complexes in Saccharomyces cerevisiae and Homo sapiens. We demonstrate that abundance levels of orthologous proteins correlate strongly between the two organisms and both networks have highly similar topologies. We therefore used the protein abundances in one species to cross‐predict missing protein abundance levels in the other species. Lastly, we identified a novel conserved low‐abundance subnetwork further demonstrating the value of quantitative analysis of networks. |
| Keywords: | human multidimensional protein identification technology quantitative proteomics topological data analysis yeast |