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Single muscle fiber proteomics reveals unexpected mitochondrial specialization |
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| Authors: | Marta Murgia Nagarjuna Nagaraj Atul S Deshmukh Marlis Zeiler Pasqua Cancellara Irene Moretti Carlo Reggiani Stefano Schiaffino Matthias Mann |
| Affiliation: | 1. Department of Proteomics and Signal Transduction, Max‐Planck‐Institute of Biochemistry, Martinsried, Germany;2. Department of Biomedical Sciences, University of Padova, Padua, Italy;3. Department of Proteomics, The Novo Nordisk Foundation Center for Protein Research, Faculty of Health Sciences, University of Copenhagen, Copenhagen, Denmark;4. Venetian Institute of Molecular Medicine, Padua, Italy |
| Abstract: | Mammalian skeletal muscles are composed of multinucleated cells termed slow or fast fibers according to their contractile and metabolic properties. Here, we developed a high‐sensitivity workflow to characterize the proteome of single fibers. Analysis of segments of the same fiber by traditional and unbiased proteomics methods yielded the same subtype assignment. We discovered novel subtype‐specific features, most prominently mitochondrial specialization of fiber types in substrate utilization. The fiber type‐resolved proteomes can be applied to a variety of physiological and pathological conditions and illustrate the utility of single cell type analysis for dissecting proteomic heterogeneity. |
| Keywords: | exercise metabolism mitochondria muscle fibers single cell |