| Abstract: | Some causative factors resulting in the appearance of two components of ferredoxin (Fd) isolated from the pea leaves preparations have been studied by 15% PAG electrophoresis. It has been shown that the composition of the buffer systems (tris-glycine buffer, pH 8,3; veronal buffer, pH 7.0) does not cause Fd separation into two components. Study of the effects of the modifying agents on the active centre and apoprotein of Fd. Showed that the destruction of the active centre does not affect Fd separation and modification of the Fd protein part results in greater amount of the bands on the electrophoregrams. It has been concluded that heterogeneity of Fd is possibly of the genetic nature. The following facts can be regarded as an evidence for existence of both forms of Fd in pea leaves: occurrence of two forms of Fd in chloroplasts isolated in organic media, differing in Fd content during pea ontogenesis and upon varying illumination conditions during their growth. |
