Neurochemical Characteristics of a Novel Dorsal Root Ganglion X Neuroblastoma Hybrid Cell Line, F-11 |
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Authors: | Paul C Francel Kathy Harris Michelle Smith Mark C Fishman Glyn Dawson†‡ Richard J Miller |
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Institution: | Department of Pharmacological and Physiological Sciences, Illinois, U.S.A.;Massachusetts General Hospital, Harvard Medical School, Boston, Massachusetts, U.S.A.;Department of Biochemistry, University of Chicago, Chicago, Illinois, U.S.A.;Department of Pediatrics, Joseph P. Kennedy, Jr. Mental Retardation Research Center, University of Chicago, Chicago, Illinois, U.S.A. |
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Abstract: | We investigated the properties of the novel dorsal root ganglion (DRG) hybrid cell line F-11 to see how closely these cells resembled normal DRG cells. Under normal growth conditions, F-11 cells appeared to contain several short neurite-like processes. However, these cells could also be grown under conditions in which they showed a much more extensive neuronal morphology, exhibiting many long neurites. Several differentiated features of DRG cells were present on F-11 cells. These included the presence of delta-opioid receptors, receptors for prostaglandins and bradykinin, and dihydropyridine-sensitive calcium channels. F-11 cells also synthesized and released a substance P-like compound, as determined by immunoreactivity. Both the number of bradykinin receptors and the voltage-sensitive calcium influx increased on cell differentiation. Opioid agonists (delta-specificity) were found to decrease cyclic AMP levels in F-11 cells in a naloxone- and pertussis toxin-reversible fashion. Bradykinin stimulated the synthesis of inositol-1,4-bisphosphate and inositol-1,4,5-trisphosphate. Ca2+ channel agonists stimulated voltage-sensitive Ca2+ influx in a dose-dependent, stereospecific manner, whereas Ca2+ channel antagonists inhibited Ca2+ influx. F-11 cells should, therefore, prove useful as models for authentic DRG neurons. |
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Keywords: | Opiate receptors Bradykinin Calcium channels Prostanoids Substance P-like immunoreactivity Dorsal root ganglion |
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