Increased activity of the non-regulated enzymes fructose-1,6-bisphosphate aldolase and triosephosphate isomerase in Anabaena sp. strain PCC 7120 increases photosynthetic yield |
| |
Authors: | Weimin Ma Lanzhen Wei Quanxi Wang Dingji Shi Haibao Chen |
| |
Institution: | (1) College of Life and Environment Sciences, Shanghai Normal University, Shanghai, 200234, People’s Republic of China;(2) Laboratory of Photosynthesis, Institute of Botany, Chinese Academy of Science, Beijing, 100093, People’s Republic of China;(3) National Laboratory of Plant Molecular Genetics, Institute of Plant Physiology and Ecology, Shanghai Institutes for Biological Sciences, Graduate School of the Chinese Academy of Sciences, 300 Fenglin Road, Shanghai, 200032, People’s Republic of China;(4) State Key Laboratory of Bio-organic and Natural Products Chemistry, Institute of Organic Chemistry, Chinese Academy of Science, Shanghai, 200032, People’s Republic of China |
| |
Abstract: | Non-regulated enzymes in the Calvin cycle are generally presumed to be less important for the regulation of photosynthetic
yield. Here, to investigate the relationship between the activity of non-regulated enzymes and photosynthetic yield, two non-regulated
enzymes in the Calvin cycle—a rice cytosolic fructose-1,6-bisphosphate aldolase (FBA) and a spinach chloroplast triosephosphate
isomerase (TPI)—were cloned and co-expressed in cells of the cyanobacterium Anabaena sp. strain PCC 7120. The activity of FBA and TPI and the photosynthetic yield reflected by photosynthetic O2 evolution and cell dry weight were measured and compared between wild-type and transgenic cells. Our results demonstrated
that the activity of FBA and TPI were increased in transgenic cells relative to wild-type cells, and that activity was further
increased in a transgenic strain harboring two sets of FBA-TPI tandem genes relative to cells containing one copy of the FBA-TPI
tandem gene. The increased activity of FBA and TPI in Anabaena sp. strain PCC 7120 increased photosynthetic yield, with increased activity levels correlating closely with the degree of
changes in photosynthetic yield. This implies that the photosynthetic yield is limited by the activity of the non-regulated
enzymes FBA and TPI, and that the endogenous activity of non-regulated enzymes is not sufficient to increase photosynthetic
yield. We discuss the various roles of FBA and TPI, and regulated and non-regulated enzymes, in modulating photosynthetic
yield.
W. Ma and L. Wei contributed equally to this work. |
| |
Keywords: | Anabaena sp strain PCC 7120 enzyme activity fructose-1 6-bisphosphate aldolase photosynthetic yield triosephosphate isomerase |
本文献已被 SpringerLink 等数据库收录! |
|