Sensitive and rapid detection of Karenia mikimotoi (Dinophyceae) by loop-mediated isothermal amplification |
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| Authors: | Fengying Zhang Lingbo Ma Zhaoli Xu Junbin Zheng Yanhong Shi Yanan Lu Yuping Miao |
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| Institution: | aEast China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Key and Open Laboratory of Marine and Estuarine Fisheries, Ministry of Agriculture of China, Shanghai 200090, China |
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| Abstract: | A loop-mediated isothermal amplification (LAMP) assay was developed to detect the genomic DNA of Karenia mikimotoi using a set of four specific primers based on a ribosomal DNA internal transcribed spacer (ITS). The sensitivity of this LAMP assay was 100-fold higher than regular PCR, and its specificity was validated using other algae as a comparison. Two visual detection approaches were feasible to interpret the positive or negative results. This technology may have the potential to aid in forecasting red-tides on the scene because of its high sensitivity, specificity and rapid detection. |
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| Keywords: | Identification Karenia mikimotoi Loop-mediated isothermal amplification (LAMP) Ribosomal DNA internal transcribed spacer (ITS) |
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