模拟微重力环境因子对人参细胞生长和人参皂苷含量的影响

与在正常重力条件培养下的对照相比，经回转器水平回转处理的人参细胞鲜重和干重均增加，人参皂苷含量提高10％左右。在去Ca2 培养基上生长的人参愈伤组织细胞，经回转器水平回转3周后，人参皂苷含量约为正常重力条件下培养细胞的2倍。另外，在试验范围内，如果培养基中起始钙离子浓度越高，则其培养的人参细胞中人参皂苷含量越低。

Influence of Simulated Microgravity Environmental Factor on Ginseng Cell Growth and Ginseng Saponin Content

When ginseng (Panax ginseng C. A. Meyer ) cells were subjectedto horizontal rotation on a clinostat, theirgrowth and ginseng saponin content differed from those cultured in normal Bothitational environments (control ). Bothfresh and dry weights of ginseng cells rotating on clinostat were higher than thoseof the control, and the difference in dryweight was particularly obvious (Fig. 1 ).After 3 weeks of cultivation, saponincontent in ginseng was 10 % higher under the horizontal rotation treatment onthe clinostat than that of the control (Fig. 3). When ginseng cells were cultured on Ca2 -deprived medium and clinostatted for 3 weeks, their ginsengsaponin content was almost twice of thatof the control (Fig. 4). Besides, in ourexperiments, the higher the Ca2 concentration in medium, the lower the ginseng saponin content in the ginseng cellscultured (Fig. 2).